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dapt 10 um  (Fisher Scientific)

 
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    Structured Review

    Fisher Scientific dapt 10 um
    a, Images of organoids from crypts with and without UK-5099. Blue arrow points to Lgr5 -EGFP + crypt domain and purple asterisks indicate autofluorescent lumens. b, Quantification of crypt domains per organoid with and without UK-5099 ( n = 6 experimental replicates). c, Quantification of organoid formation from crypts from proximal small intestine with and without UK-5099 ( n = 16 wENR, n= 14 UK-5099 experimental replicates). d, Flow cytometry for cells in (c) showing percent GFP positive ( n = 3 replicates per condition). e, Human ascending colon crypts treated with wENR basal media (set to 1) or media containing UK-5099 or CHIR+VAL ( n = 5 patients with 6–8 individual cultures per patient sample). f, Representative images and quantification of crypt domains in organoids maintained with and without UK-5099 for 12 months (wENR n = 46 organoids from 2 separate wells, UK-5099 n = 26 organoids from 2 separate wells). g, Images of organoids treated <t>with</t> <t>IWP-2</t> and <t>DAPT</t> to promote differentiation with and without UK-5099. Blue arrows point to crypt domains and yellow asterisks indicate autofluorescent lumens. h, Organoid formation from crypts treated with the indicated single, double, and triple drug combinations (CHIR = CHIR99021 and VAL = valproic acid) ( n = 10 experimental replicates, p values are for each treatment compared to wENR). i, Transcript abundance in organoids showing the ratio of UK5099 treated to control ( n = 3 experimental replicates, p values calculated for each transcript compared to wENR). j, Heat map for additional transcripts from (i) . k, Probability of cells infected with virus containing empty vector or both MPC1-iRFP and MPC2-mCherry also being GFP positive ( n = 4 independent organoids with at least 15 infected cells counted). Data are mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars, 50 μm ( a ,) and 100 μm ( f, g ). P values for 6h were calculated by One-way ANOVA with correction for multiple comparisons. All other p values were calculated using Student’s t -test.
    Dapt 10 Um, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapt 10 um/product/Fisher Scientific
    Average 90 stars, based on 1 article reviews
    dapt 10 um - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism"

    Article Title: Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism

    Journal: Nature cell biology

    doi: 10.1038/ncb3593

    a, Images of organoids from crypts with and without UK-5099. Blue arrow points to Lgr5 -EGFP + crypt domain and purple asterisks indicate autofluorescent lumens. b, Quantification of crypt domains per organoid with and without UK-5099 ( n = 6 experimental replicates). c, Quantification of organoid formation from crypts from proximal small intestine with and without UK-5099 ( n = 16 wENR, n= 14 UK-5099 experimental replicates). d, Flow cytometry for cells in (c) showing percent GFP positive ( n = 3 replicates per condition). e, Human ascending colon crypts treated with wENR basal media (set to 1) or media containing UK-5099 or CHIR+VAL ( n = 5 patients with 6–8 individual cultures per patient sample). f, Representative images and quantification of crypt domains in organoids maintained with and without UK-5099 for 12 months (wENR n = 46 organoids from 2 separate wells, UK-5099 n = 26 organoids from 2 separate wells). g, Images of organoids treated with IWP-2 and DAPT to promote differentiation with and without UK-5099. Blue arrows point to crypt domains and yellow asterisks indicate autofluorescent lumens. h, Organoid formation from crypts treated with the indicated single, double, and triple drug combinations (CHIR = CHIR99021 and VAL = valproic acid) ( n = 10 experimental replicates, p values are for each treatment compared to wENR). i, Transcript abundance in organoids showing the ratio of UK5099 treated to control ( n = 3 experimental replicates, p values calculated for each transcript compared to wENR). j, Heat map for additional transcripts from (i) . k, Probability of cells infected with virus containing empty vector or both MPC1-iRFP and MPC2-mCherry also being GFP positive ( n = 4 independent organoids with at least 15 infected cells counted). Data are mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars, 50 μm ( a ,) and 100 μm ( f, g ). P values for 6h were calculated by One-way ANOVA with correction for multiple comparisons. All other p values were calculated using Student’s t -test.
    Figure Legend Snippet: a, Images of organoids from crypts with and without UK-5099. Blue arrow points to Lgr5 -EGFP + crypt domain and purple asterisks indicate autofluorescent lumens. b, Quantification of crypt domains per organoid with and without UK-5099 ( n = 6 experimental replicates). c, Quantification of organoid formation from crypts from proximal small intestine with and without UK-5099 ( n = 16 wENR, n= 14 UK-5099 experimental replicates). d, Flow cytometry for cells in (c) showing percent GFP positive ( n = 3 replicates per condition). e, Human ascending colon crypts treated with wENR basal media (set to 1) or media containing UK-5099 or CHIR+VAL ( n = 5 patients with 6–8 individual cultures per patient sample). f, Representative images and quantification of crypt domains in organoids maintained with and without UK-5099 for 12 months (wENR n = 46 organoids from 2 separate wells, UK-5099 n = 26 organoids from 2 separate wells). g, Images of organoids treated with IWP-2 and DAPT to promote differentiation with and without UK-5099. Blue arrows point to crypt domains and yellow asterisks indicate autofluorescent lumens. h, Organoid formation from crypts treated with the indicated single, double, and triple drug combinations (CHIR = CHIR99021 and VAL = valproic acid) ( n = 10 experimental replicates, p values are for each treatment compared to wENR). i, Transcript abundance in organoids showing the ratio of UK5099 treated to control ( n = 3 experimental replicates, p values calculated for each transcript compared to wENR). j, Heat map for additional transcripts from (i) . k, Probability of cells infected with virus containing empty vector or both MPC1-iRFP and MPC2-mCherry also being GFP positive ( n = 4 independent organoids with at least 15 infected cells counted). Data are mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars, 50 μm ( a ,) and 100 μm ( f, g ). P values for 6h were calculated by One-way ANOVA with correction for multiple comparisons. All other p values were calculated using Student’s t -test.

    Techniques Used: Flow Cytometry, Control, Infection, Virus, Plasmid Preparation



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    a, Images of organoids from crypts with and without UK-5099. Blue arrow points to Lgr5 -EGFP + crypt domain and purple asterisks indicate autofluorescent lumens. b, Quantification of crypt domains per organoid with and without UK-5099 ( n = 6 experimental replicates). c, Quantification of organoid formation from crypts from proximal small intestine with and without UK-5099 ( n = 16 wENR, n= 14 UK-5099 experimental replicates). d, Flow cytometry for cells in (c) showing percent GFP positive ( n = 3 replicates per condition). e, Human ascending colon crypts treated with wENR basal media (set to 1) or media containing UK-5099 or CHIR+VAL ( n = 5 patients with 6–8 individual cultures per patient sample). f, Representative images and quantification of crypt domains in organoids maintained with and without UK-5099 for 12 months (wENR n = 46 organoids from 2 separate wells, UK-5099 n = 26 organoids from 2 separate wells). g, Images of organoids treated <t>with</t> <t>IWP-2</t> and <t>DAPT</t> to promote differentiation with and without UK-5099. Blue arrows point to crypt domains and yellow asterisks indicate autofluorescent lumens. h, Organoid formation from crypts treated with the indicated single, double, and triple drug combinations (CHIR = CHIR99021 and VAL = valproic acid) ( n = 10 experimental replicates, p values are for each treatment compared to wENR). i, Transcript abundance in organoids showing the ratio of UK5099 treated to control ( n = 3 experimental replicates, p values calculated for each transcript compared to wENR). j, Heat map for additional transcripts from (i) . k, Probability of cells infected with virus containing empty vector or both MPC1-iRFP and MPC2-mCherry also being GFP positive ( n = 4 independent organoids with at least 15 infected cells counted). Data are mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars, 50 μm ( a ,) and 100 μm ( f, g ). P values for 6h were calculated by One-way ANOVA with correction for multiple comparisons. All other p values were calculated using Student’s t -test.
    Dapt 10 Um, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapt 10 um/product/Fisher Scientific
    Average 90 stars, based on 1 article reviews
    dapt 10 um - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a, Images of organoids from crypts with and without UK-5099. Blue arrow points to Lgr5 -EGFP + crypt domain and purple asterisks indicate autofluorescent lumens. b, Quantification of crypt domains per organoid with and without UK-5099 ( n = 6 experimental replicates). c, Quantification of organoid formation from crypts from proximal small intestine with and without UK-5099 ( n = 16 wENR, n= 14 UK-5099 experimental replicates). d, Flow cytometry for cells in (c) showing percent GFP positive ( n = 3 replicates per condition). e, Human ascending colon crypts treated with wENR basal media (set to 1) or media containing UK-5099 or CHIR+VAL ( n = 5 patients with 6–8 individual cultures per patient sample). f, Representative images and quantification of crypt domains in organoids maintained with and without UK-5099 for 12 months (wENR n = 46 organoids from 2 separate wells, UK-5099 n = 26 organoids from 2 separate wells). g, Images of organoids treated with IWP-2 and DAPT to promote differentiation with and without UK-5099. Blue arrows point to crypt domains and yellow asterisks indicate autofluorescent lumens. h, Organoid formation from crypts treated with the indicated single, double, and triple drug combinations (CHIR = CHIR99021 and VAL = valproic acid) ( n = 10 experimental replicates, p values are for each treatment compared to wENR). i, Transcript abundance in organoids showing the ratio of UK5099 treated to control ( n = 3 experimental replicates, p values calculated for each transcript compared to wENR). j, Heat map for additional transcripts from (i) . k, Probability of cells infected with virus containing empty vector or both MPC1-iRFP and MPC2-mCherry also being GFP positive ( n = 4 independent organoids with at least 15 infected cells counted). Data are mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars, 50 μm ( a ,) and 100 μm ( f, g ). P values for 6h were calculated by One-way ANOVA with correction for multiple comparisons. All other p values were calculated using Student’s t -test.

    Journal: Nature cell biology

    Article Title: Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism

    doi: 10.1038/ncb3593

    Figure Lengend Snippet: a, Images of organoids from crypts with and without UK-5099. Blue arrow points to Lgr5 -EGFP + crypt domain and purple asterisks indicate autofluorescent lumens. b, Quantification of crypt domains per organoid with and without UK-5099 ( n = 6 experimental replicates). c, Quantification of organoid formation from crypts from proximal small intestine with and without UK-5099 ( n = 16 wENR, n= 14 UK-5099 experimental replicates). d, Flow cytometry for cells in (c) showing percent GFP positive ( n = 3 replicates per condition). e, Human ascending colon crypts treated with wENR basal media (set to 1) or media containing UK-5099 or CHIR+VAL ( n = 5 patients with 6–8 individual cultures per patient sample). f, Representative images and quantification of crypt domains in organoids maintained with and without UK-5099 for 12 months (wENR n = 46 organoids from 2 separate wells, UK-5099 n = 26 organoids from 2 separate wells). g, Images of organoids treated with IWP-2 and DAPT to promote differentiation with and without UK-5099. Blue arrows point to crypt domains and yellow asterisks indicate autofluorescent lumens. h, Organoid formation from crypts treated with the indicated single, double, and triple drug combinations (CHIR = CHIR99021 and VAL = valproic acid) ( n = 10 experimental replicates, p values are for each treatment compared to wENR). i, Transcript abundance in organoids showing the ratio of UK5099 treated to control ( n = 3 experimental replicates, p values calculated for each transcript compared to wENR). j, Heat map for additional transcripts from (i) . k, Probability of cells infected with virus containing empty vector or both MPC1-iRFP and MPC2-mCherry also being GFP positive ( n = 4 independent organoids with at least 15 infected cells counted). Data are mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars, 50 μm ( a ,) and 100 μm ( f, g ). P values for 6h were calculated by One-way ANOVA with correction for multiple comparisons. All other p values were calculated using Student’s t -test.

    Article Snippet: To promote robust differentiation IWP-2 (2 uM Fisher Scientific #35–331-0) and DAPT (10 uM Fisher Scientific #26–341-0) were added to organoid media along with vehicle (DMSO) or UK-5099 (20 uM).

    Techniques: Flow Cytometry, Control, Infection, Virus, Plasmid Preparation